WebFigure Legend Snippet: a Immunofluorescence of BG4 and fluorescence in situ hybridization (FISH) for viral genomic RNA (gRNA) in Vero E6 cells with or without ARS-CoV-2 infection. Scale bars, 10 μm. In the inset of selected regions, the colocalized foci of gRNA (red) with BG4 (green) are indicated by white arrows. WebMar 12, 2024 · EGFP-SctQ remained localized in foci representing assembled cytosolic complexes at pH 6.3 and 6.0 (Fig. 3b), indicating that the observed disassembly of the cytosolic complex is not caused by the ...
G-quadruplexes mark alternative lengthening of telomeres
WebThe cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antibody fo... View more Product Details Product Specific Information Product will be shipped at Room Temperature. Target Information WebNov 26, 2024 · Cyclic GMP-AMP synthase (cGAS) is a major sensor of cytosolic DNA from invading pathogens and damaged cellular organelles. Activation of cGAS promotes liquid-like phase separation and formation of membraneless cytoplasmic structures. Here, we found that cGAS bound G3BP1, a double-stranded nucleic ac … phoenix foundry motifs
Development of a novel light-up probe for detection of G …
WebJun 29, 2024 · 这些G4形成主要集中在染色质开放区域及癌基因的启动子区域,并与转录紧密相关。 并且,它们有偏向性的在一些特定亚型乳腺癌组织中形成。 此外,不同功能的蛋白能特异地结合G4。 由此可知,体内G4动态形成及其功能很可能受到不同类型蛋白质的调控。 因此,开发系统性分析G4相互作用蛋白质的方法,不仅能让我们从分子层面了解其作用 … WebDec 3, 2024 · Recently, an antibody, BG4, was described to study G4 structures within cells. Here, we characterize BG4 for its affinity towards G4-DNA, using several biochemical … WebFeb 2, 2016 · Telomestatin stabilizes G-quadruplexes (G4s), which are guanine-rich four-strand nucleic acid structures observed in vitroand in vivo. However, the mechanism underlying the GSC-selective nature of the DNA damage response remains unknown. phoenix foundry emblems