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Lamp2 staining

Tīmeklis2024. gada 3. maijs · Cells were fixed and stained for nuclei (Hoechst) and lysosomes (LAMP2), as described under “immunocytochemistry” (Supplementary file 1). The plates were imaged using ImageXpress Micro Confocal in widefield mode, using a 40x air objective. Hoechst immunostaining was captured using a DAPI filter and LAMP2 …

Anti-LAMP2A antibody - Lysosome Marker …

Tīmeklis2016. gada 4. marts · In our case of a new variant of CTE, as expected, immunostaining for EpCAM was absent from the basolateral membrane, 7 but in … TīmeklisCD107b which is also known as Mac-3, Lysosome-associated membrane protein 2 (LAMP-2/Lamp2/Lamp II), and Lysosomal membrane glycoprotein type B (LGP-B). Cite 1 Recommendation 26th Apr, 2024 Julie... health finger ring https://ohiodronellc.com

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Tīmeklis2024. gada 22. sept. · LAMP2 is a transmembrane glycoprotein in the lysosomal membrane with three splice variants (LAMP2A, B, and C). The three isoforms share … TīmeklisStaining pattern of p62, LC3 and LAMP2 in IMNM and sIBM. LC3 (A, B) and p62 (D, E) stains in IMNM skeletal muscle biopsies show a fine granular pattern throughout the entire sarcoplasm with... Tīmeklis2024. gada 23. marts · For GRP78 and LAMP2 staining, the oocytes after blocking were incubated with rabbit polyclonal anti-LAMP2 antibody and rabbit polyclonal anti-GRP78 (1:100) at 4°C overnight, and then the oocytes were washed by wash buffer (0.1% Tween 20 and 0.01% Triton X-100) three times (2 min each time). Next the … health fingertips

Ferroptosis-Related Prognostic Gene LAMP2 Is a Potential …

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Lamp2 staining

LAMP2 Polyclonal Antibody Invitrogen PA1-655 product …

TīmeklisLAMP1 and LAMP2 glycoproteins comprise 50% of all lysosomal membrane proteins, and are thought to be responsible in part for maintaining lysosomal … TīmeklisDue to enhanced expression of LAMP2 glycoproteins caused by LAMP1 gene deficits, LAMP1 and LAMP2 expression are related (11). Therefore, it is believed that the two have comparable in vivo functions.

Lamp2 staining

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TīmeklisLAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C. Database link: P13473 (Peptide available as ab23322) Positive control WB: Human liver tissue lysate. ICC: CaCo2 cells, HeLa, MCF7 and HepG2 cells. … TīmeklisThe cells were stained with 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) (1:1000, Beyotime, Hangzhou, China) for 5 mins and mounted on slides. Immunofluorescence images were captured by a fluorescence microscope (BX51, Olympus, Japan). ... Immunohistochemical staining showed that PHLPP and …

TīmeklisLAMP2. On the top, protein expression in current human tissue, based on all annotated cell types, is reported with the units not detected (n), low (l), medium (m) and high … Tīmeklis2024. gada 21. febr. · LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome.

Tīmeklis2024. gada 3. sept. · Neurons at DIV7 were stained with BODIPY–pepstatin A (1 µM) for 1 h followed by fixation and immunostaining with antibodies against LAMP1 and CI-M6PR. White arrows indicate LAMP1-labeled organelles with active cathepsin D, orange arrows point to nondegradative LAMP1 organelles that are costained by the late … Tīmeklis2024. gada 12. nov. · Lysosome-associated membrane protein type 2A (LAMP2A) is a key protein in the chaperone-mediated autophagy (CMA) pathway. LAMP2A …

Tīmeklis2024. gada 10. apr. · d, Wild-type or RagA and RagB DKO HEK293T cells expressing Ctrl or ILF3 shRNAs were treated as in a, immunostained with antibodies against mTOR (red) and LAMP2 (green), co-stained with DAPI (blue ...

TīmeklisLAMP2. On the top, protein expression in current human tissue, based on all annotated cell types, is reported with the units not detected (n), low (l), medium (m) and high … gonsalves red wine vinegarTīmeklis2024. gada 14. maijs · Staining with Lysotracker showed that LLOMe exposure initially reduced Lysotracker-positive lysosomes, as a sign of lysosomal membrane damage. … gonsalves service stationTīmeklisProceed with staining or store cells at 4°C in PBS overnight. C. Immunostaining. NOTE: Count cells using a hemocytometer or alternative method. Aliquot desired number of cells into tubes or wells. ... (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily … health finder washington stateTīmeklisSPLEEN - Antibody staining i Antibody staining in the annotated cell types in the current human tissue is reported as not detected, low, medium, or high, based on … health first 100 church st new york nyTīmeklisSKIN 2 - Antibody staining i Antibody staining in the annotated cell types in the current human tissue is reported as not detected, low, medium, or high, based on … health fire reactivity labelsTīmeklis2024. gada 4. jūl. · Immunohistochemical staining validated LAMP2 expression in prostate tissue (both normal and cancerous tissues) with cytoplasmic or membranous location (Figures 6(c)–6(e)). Then, we conducted survival analysis for LAMP2 in different subgroups of prostate cancer while no significant association was detected (Figure 6(f)). health fingernails ridgesTīmeklis2024. gada 20. jūl. · Alternatively, at 168 h after ischemia, LAMP2 staining mainly existed in cells which exhibited the typical features of activated microglia ( Figure 3A, e–h ). The results from double-fluorescent... health fingerplays