Psh65 plasmid
WebBackground: Adherence to the surface of the host cell is the precondition for T. vaginalis parasitism and pathogenicity, causing urogenital infection. The AP65 of T. vaginalis … Webthe plasmid pUG6 as a template (Gueldener et al. 2002). Saccharomyces cerevisiae cells were trans- ... containing 300 lg G418/ml. The G418-resistant KanMX gene was excised with Cre recom-binase and expressed from the pSH65 plasmid (Gueldener et al. 2002). Heterogeneous expression of JJJ1 in BYDJJJ1 was performed by cloning the JJJ1 ORF …
Psh65 plasmid
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WebMar 15, 2002 · For construction of plasmid pSH65 ( GAL1-cre, ble) the vector pUT332, containing the bler gene from transposon Tn 5 plus the S.cerevisiaeTEF1 promoter ( 34 ), … WebThe present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene …
WebStudy population. One hundred and ten consecutive patients undergoing allograft transplantation at Nantes University Hospital and at risk of CMV active infection were … WebDec 1, 2024 · Schizochytrium sp. is commercially used for the production of docosahexaenoic acid (DHA). Some strains of Schizochytrium sp. are also known to produce low amounts of carotenoids, including...
WebpSH65 plasmid,pSH65,pSH65 plasmid,pSH65 sequence,pSH65 map WebApr 11, 2016 · The G418-resistant KanMX gene was excised with Cre recombinase and expressed from the pSH65 plasmid (Gueldener et al. 2002 ). Heterogeneous expression of JJJ1 in BYΔJJJ1 was performed by cloning the JJJ1 ORF into the EcoR I and Kpn I sites of the pYES2 plasmid (Invitrogen, Carlsbad, CA, USA).
WebApr 30, 2016 · The yeast Saccharomyces cerevisiae is an important eukaryotic workhorse in traditional and modern biotechnology. At present, only a few S. cerevisiae strains have been extensively used as engineering hosts. Recently, an astonishing genotypic and phenotypic diversity of S. cerevisiae was disclosed in natural populations.
WebOct 8, 2013 · pSH65 (P30122) deletion marker Reference: Güldener U, Heinisch J, Köhler GJ, Voss D, Hegemann JH. A second set of loxP marker cassettes for Cre-mediated multiple … connection error:invalid user id or passwordWebOct 26, 2012 · by transformation with the pSH65 plasmid, expressing the induc-ible Cre recombinase and carrying the phleomycin resistance gene. ble. r (15). Loss of pSH65 was achieved by growing cells in liquid ... connection error asphalt 9WebDear colleagues, friends, can everyone exchange plasmids?Our laboratory now needs two plasmids: PUG6, PSH65.There must be PFA6A -KANMX4.Our laboratory now has … edinburgh music centreWebApr 14, 2024 · In addition, using the electrotransformation to remove the selected marker KanMX [ 32 ], the pSH65 plasmid was transformed into haploid yeast strains with LEU1 and PDC5 deletions, and finally, the recombinant yeast strains XFa7-P/XFα6-P and XFa7-L/XFα6-L were obtained. edinburgh music festival augustWebConstruction of plasmid pCU563 carrying a heterologous selection-marker gene flanked by loxP sites. A DNA fragment containing 34-bp loxP, the 0.45-kb PGKpromoter ( pr), and the … edinburgh musicals 2021WebPlasmid: pSH. Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse … connection error. is pipelines runningWebJul 1, 2013 · Loss of pSH65 was achieved by growing cells in liquid YPD medium at 30 °C for 2 days. 2.3. Determination of extracellular inulinase activity Recombinants were grown in 50 mL of YPI medium (2% peptone, 1% yeast extract and 2% inulin [w/v], pH 5.5) at 30 °C with shaking at 200 rpm. edinburgh music festival 2020